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PHC3 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - PHC3 Antibody (C-Term) AP21862b
    AP21862b staining PHC3 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - PHC3 Antibody (C-Term) AP21862b
    All lanes : Anti-PHC3 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: A549 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 106 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q8NDX5
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 106162 Da
Additional Information
Gene ID 80012
Other Names Polyhomeotic-like protein 3, Early development regulatory protein 3, Homolog of polyhomeotic 3, hPH3, PHC3, EDR3, PH3
Target/Specificity This PHC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 819-850 amino acids from human PHC3.
Dilution IHC-P~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHC3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHC3
Synonyms EDR3, PH3
Function Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Cellular Location Nucleus
Research Areas
Citations (0)

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Background

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

References

Tonkin E.,et al.Hum. Genet. 111:435-442(2002).
Levine S.S.,et al.Mol. Cell. Biol. 22:6070-6078(2002).
Hansen M.F.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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$ 295.00
$ 99.00
Cat# AP21862b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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