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GNG12 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GNG12 Antibody (C-Term) AP21906b
    All lanes : Anti-GNG12 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 8 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - GNG12 Antibody (C-Term) AP21906b
    AP21906b staining GNG12 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9UBI6
Other Accession Q28024, Q9DAS9, Q5RBQ0
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 8006 Da
Antigen Region 37-68 aa
Additional Information
Gene ID 55970
Other Names Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12, GNG12
Target/Specificity This GNG12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-68 amino acids from human GNG12.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNG12 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNG12
Function Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Research Areas
Citations (0)
citation

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Background

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.

References

Hurowitz E.H.,et al.DNA Res. 7:111-120(2000).
Yang L.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Cook L.A.,et al.Protein Sci. 10:2548-2555(2001).
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP21906b
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