|Application ||WB, E|
|Calculated MW||36179 Da|
|Other Names||DNA-binding death effector domain-containing protein 2, DED-containing protein FLAME-3, FADD-like anti-apoptotic molecule 3, DEDD2, FLAME3|
|Target/Specificity||This DEDD2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 162-193 amino acids from the Central region of human DEDD2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DEDD2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3.|
|Cellular Location||Nucleus, nucleolus. Note=Nuclear, accumulated in subnuclear structures resembling nucleoli|
|Tissue Location||Expressed in most tissues. High levels were found in liver, kidney, heart, ovary, spleen, testes, skeletal muscle and peripheral blood leukocytes. Expression was absent or low in colon and small intestine. Expression is relatively high in the tumor cell lines chronic myologenous leukemia K-562 and the colorectal adenocarcinoma SW480. Expression is moderate in the cervical carcinoma HeLa, the Burkitt's lymphoma Raji, the lung carcinoma A-549, and the melanoma G-361. In contrast, two leukemia cell lines, HL-60 (promyelocytic leukemia) and MOLT-4 (lymphoblastic leukemia), show relatively low levels|
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May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3.
Roth W.,et al.J. Biol. Chem. 277:7501-7508(2002).
Zhan Y.,et al.Cell Death Differ. 9:439-447(2002).
Lee J.C.,et al.J. Cell Biol. 158:1051-1066(2002).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Alcivar A.,et al.Oncogene 22:291-297(2003).
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