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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PSME4 Antibody (N-Term)   

PSME4 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PSME4 Antibody (N-Term) AP21982a
    All lanes : Anti-PSME4 Antibody (N-Term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 211 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q14997
Other Accession F1MKX4
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 211334 Da
Additional Information
Gene ID 23198
Other Names Proteasome activator complex subunit 4, Proteasome activator PA200, PSME4, KIAA0077
Target/Specificity This PSME4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 503-535 amino acids from human PSME4.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSME4 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSME4
Synonyms KIAA0077
Function Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin- independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.
Cellular Location Cytoplasm, cytosol. Nucleus. Nucleus speckle Note=Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2)O(2)
Research Areas
Citations (0)

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Background

Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin- independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.

References

Blickwedehl J.,et al.Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
Nomura N.,et al.DNA Res. 1:223-229(1994).
Ustrell V.,et al.EMBO J. 21:3516-3525(2002).
Wang X.,et al.Biochemistry 46:3553-3565(2007).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

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$ 295.00
$ 99.00
Cat# AP21982a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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