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NAA10 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - NAA10 Antibody (Center) AP21992c
    All lanes : Anti-NAA10 Antibody (Center) at 1:2000 dilution Lane 1: SK-BR-3 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HCT116 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P41227
Other Accession Q2KI14, Q9QY36
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 26459 Da
Additional Information
Gene ID 8260
Other Names N-alpha-acetyltransferase 10, 2.3.1.-, 2.3.1.88, N-terminal acetyltransferase complex ARD1 subunit homolog A, NatA catalytic subunit Naa10, NAA10, ARD1, ARD1A, TE2
Target/Specificity This NAA10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 135-167 amino acids from the Central region of human NAA10.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNAA10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NAA10
Synonyms ARD1, ARD1A, TE2
Function Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development.
Cellular Location Cytoplasm. Nucleus. Note=Also present in the free cytosolic and cytoskeleton-bound polysomes
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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Background

Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development.

References

Tribioli C.,et al.Hum. Mol. Genet. 3:1061-1068(1994).
Arnesen T.,et al.Biochem. J. 386:433-443(2005).
Ross M.T.,et al.Nature 434:325-337(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Jeong J.-W.,et al.Cell 111:709-720(2002).

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$ 295.00
$ 99.00
Cat# AP21992c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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