|Application ||FC, WB, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||116717 Da|
|Other Names||Vinculin, Metavinculin, Vcl|
|Target/Specificity||This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 634-668 amino acids from mouse.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Vinculin is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Note=Cytoplasmic face of adhesion plaques Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. Co-localizes with LIMD1 in the focal adhesions (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).
Coll J.-L.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:9161-9165(1995).
Alatortsev V.E.,et al.FEBS Lett. 413:197-201(1997).
Carninci P.,et al.Science 309:1559-1563(2005).
Lubec G.,et al.Submitted (JAN-2009) to UniProtKB.
Mandai K.,et al.J. Cell Biol. 144:1001-1017(1999).
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