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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Vinculin   


Purified Rabbit Polyclonal Antibody (Pab)

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  • FC - Vinculin AP22112a
    Overlay histogram showing NIH/3T3 cells stained with AP22112a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22112a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - Vinculin AP22112a
    All lanes : Anti-Vinculin at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: mouse kidney lysate Lane 5: PC-12 whole cell lysate Lane 6: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 117 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q64727
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 116717 Da
Additional Information
Gene ID 22330
Other Names Vinculin, Metavinculin, Vcl
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 634-668 amino acids from mouse.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVinculin is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Vcl
Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Note=Cytoplasmic face of adhesion plaques Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. Co-localizes with LIMD1 in the focal adhesions (By similarity).
Research Areas
Citations (0)

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Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).


Coll J.-L.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:9161-9165(1995).
Alatortsev V.E.,et al.FEBS Lett. 413:197-201(1997).
Carninci P.,et al.Science 309:1559-1563(2005).
Lubec G.,et al.Submitted (JAN-2009) to UniProtKB.
Mandai K.,et al.J. Cell Biol. 144:1001-1017(1999).

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$ 295.00
$ 99.00
Cat# AP22112a
(40 western blots)
Availability: In Stock
Bulk Size
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