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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Transferrin Antibody   

Transferrin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - Transferrin Antibody AP22124a
    AP22124a staining Transferrin in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - Transferrin Antibody AP22124a
    All lanes : Anti-Transferrin Antibody at 1:2000 dilution Lane 1: human liver lysate Lane 2: human fetal liver lysate Lane 3: human plasma lysate Lane 4: HepG2 whole cell lysate Lane 5: human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P02787
Other Accession A5A6I6
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 77064 Da
Additional Information
Gene ID 7018
Other Names Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin, TF
Target/Specificity This Transferrin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 432-466 amino acids from human Transferrin.
Dilution IHC-P~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTransferrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TF
Function Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Cellular Location Secreted.
Tissue Location Expressed by the liver and secreted in plasma.
Research Areas
Citations (0)

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Background

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

References

Yang F.,et al.Proc. Natl. Acad. Sci. U.S.A. 81:2752-2756(1984).
Schaeffer E.,et al.Gene 56:109-116(1987).
Hershberger C.L.,et al.Ann. N. Y. Acad. Sci. 646:140-154(1991).
Beutler E.,et al.Blood 96:4071-4074(2000).
Muzny D.M.,et al.Nature 440:1194-1198(2006).

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$ 295.00
$ 99.00
Cat# AP22124a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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