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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   BCDIN3D Antibody (C-Term)   

BCDIN3D Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BCDIN3D Antibody (C-Term) AP22145b
    All lanes : Anti-BCDIN3D Antibody (C-Term) at 1:1000-2000 dilution Lane 1: A431 whole cell lysate Lane 2: human cerebellum lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7Z5W3
Other Accession Q5RFI3
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 33200 Da
Additional Information
Gene ID 144233
Other Names Pre-miRNA 5'-monophosphate methyltransferase, 2.1.1.-, BCDIN3 domain-containing protein, BCDIN3D
Target/Specificity This BCDIN3D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from human BCDIN3D.
Dilution WB~~1:1000-2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCDIN3D Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCDIN3D
Function O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre- miRNAs.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre- miRNAs.

References

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Xhemalce B.,et al.Cell 151:278-288(2012).

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$ 295.00
$ 99.00
Cat# AP22145b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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