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ST8SIA1 Antibody (N-Term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | Q92185 |
| Other Accession | P61642 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 40519 Da |
| Gene ID | 6489 |
|---|---|
| Other Names | Alpha-N-acetylneuraminide alpha-2, 8-sialyltransferase, 2.4.99.8, Alpha-2, 8-sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, Sialyltransferase 8A, SIAT8-A, Sialyltransferase St8Sia I, ST8SiaI, ST8SIA1, SIAT8, SIAT8A |
| Target/Specificity | This ST8SIA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 53-84 amino acids from human ST8SIA1. |
| Dilution | WB~~1:1000-1:2000 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ST8SIA1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ST8SIA1 (HGNC:10869) |
|---|---|
| Synonyms | SIAT8, SIAT8A |
| Function | Catalyzes the addition of sialic acid in alpha 2,8-linkage to the sialic acid moiety of the ganglioside GM3 to form ganglioside GD3; gangliosides are a subfamily of complex glycosphingolipds that contain one or more residues of sialic acid (PubMed:7937974, PubMed:8058740, PubMed:8195250, PubMed:8631981, PubMed:8706663, PubMed:18348864, PubMed:22885356). Can catalyze the addition of a second alpha-2,8- sialic acid to GD3 to form GT3 (PubMed:8631981). Can use GM1b, GD1a and GT1b as acceptor substrates to synthesize GD1c, GT1a and GQ1b respectively (PubMed:8706663). Can synthesize unusual tetra- and pentasialylated lactosylceramide derivatives identified as GQ3 (II3Neu5Ac4-Gg2Cer) and GP3 (II3Neu5Ac5-Gg2Cer) in breast cancer cells (PubMed:22885356). |
| Cellular Location | Golgi apparatus membrane; Single- pass type II membrane protein |
| Tissue Location | Strongly expressed in melanoma cell lines, adult and fetal brain and to a lesser extent in adult and fetal lung |
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Provided below are standard protocols that you may find useful for product applications.
Background
Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid.
References
Sasaki K.,et al.J. Biol. Chem. 269:15950-15956(1994).
Nara K.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:7952-7956(1994).
Haraguchi M.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:10455-10459(1994).
Nakayama J.,et al.J. Biol. Chem. 271:3684-3691(1996).
Rimoldi S.,et al.Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
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