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SEC24B Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SEC24B Antibody (C-Term) AP22320b
    Anti-SEC24B Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 137 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O95487
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 137418 Da
Additional Information
Gene ID 10427
Other Names Protein transport protein Sec24B, SEC24-related protein B, SEC24B
Target/Specificity This SEC24B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1209-1239 amino acids from the human region of human SEC24B.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSEC24B Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SEC24B (HGNC:10704)
Function Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed:17499046, PubMed:20427317, PubMed:18843296). Plays a central role in cargo selection within the COPII complex and together with SEC24A may have a different specificity compared to SEC24C and SEC24D. May package preferentially cargos with cytoplasmic DxE or LxxLE motifs and may also recognize conformational epitopes (PubMed:17499046, PubMed:18843296).
Cellular Location Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol
Research Areas
Citations (0)

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Background

Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.

References

Pagano A.,et al.J. Biol. Chem. 274:7833-7840(1999).
Hillier L.W.,et al.Nature 434:724-731(2005).
Olsen J.V.,et al.Cell 127:635-648(2006).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).

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$ 295.00
$ 99.00
Cat# AP22320b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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