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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   METTL14 Antibody (N-Term)   

METTL14 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - METTL14 Antibody (N-Term) AP22363a
    All lanes : Anti-METTL14 Antibody (N-Term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9HCE5
Other Accession A4IFD8, Q3UIK4, Q5R5N4
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 52150 Da
Additional Information
Gene ID 57721
Other Names N6-adenosine-methyltransferase subunit METTL14, 2.1.1.62, Methyltransferase-like protein 14, METTL14, KIAA1627
Target/Specificity This METTL14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the human region of human METTL14.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMETTL14 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name METTL14 (HGNC:29330)
Function The METTL3-METTL14 heterodimer forms a N6- methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA- binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27373337, PubMed:27281194). N6- methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity).
Cellular Location Nucleus
Research Areas
Citations (0)

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Background

N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and self-renewal of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing and processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding developmental regulators, results in transcript destabilization, maintaining the groung state of ESCs, thereby promoting self-renewal of ESCs.

References

Nagase T.,et al.DNA Res. 7:273-281(2000).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP22363a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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