- CITATIONS: 1
|Application ||WB, IHC-P, FC, E|
|Calculated MW||20878 Da|
|Antigen Region||155-184 aa|
|Other Names||ADP-ribosylation factor-like protein 2, ARL2|
|Target/Specificity||This ARL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-184 amino acids from the C-terminal region of human ARL2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ARL2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle.|
|Cellular Location||Mitochondrion intermembrane space. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cytoplasm. Note=The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria. The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria (By similarity). Not detected in the Golgi, nucleus and on the mitotic spindle. Centrosome-associated throughout the cell cycle Not detected to interphase microtubules.|
Provided below are standard protocols that you may find useful for product applications.
The ADP-ribosylation factor (ARF) genes are small GTP-binding proteins of the RAS superfamily. ARL2 is a member of a functionally distinct group of ARF-like genes. This protein is a component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine.
Antoshechkin, I., et al., Dev. Cell 2(5):579-591 (2002).
Bhamidipati, A., et al., J. Cell Biol. 149(5):1087-1096 (2000).
Guru, S.C., et al., Genome Res. 7(7):725-735 (1997).
Clark, J., et al., Proc. Natl. Acad. Sci. U.S.A. 90(19):8952-8956 (1993).
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