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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SAE1 (AOS1) Antibody (C-term)   

SAE1 (AOS1) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SAE1 (AOS1) Antibody (C-term) AP2511b
    The AOS1 C-term Pab (Cat. #AP2511b) is used in Western blot to detect AOS1 in mouse heart tissue lysate.
  • WB - SAE1 (AOS1) Antibody (C-term) AP2511b
    Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (Cat.#AP2511b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).
  • IHC-P - SAE1 (AOS1) Antibody (C-term) AP2511b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - SAE1 (AOS1) Antibody (C-term) AP2511b
    AOS1 Antibody (V315) (Cat. #AP2511b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UBE0
Other Accession Q6AXQ0, Q9R1T2, A2VE14
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38450 Da
Antigen Region 300-329 aa
Additional Information
Gene ID 10055
Other Names SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SUMO-activating enzyme subunit 1, N-terminally processed, SAE1, AOS1, SUA1, UBLE1A
Target/Specificity This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the C-terminal region of human SAE1 (AOS1).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSAE1 (AOS1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SAE1
Synonyms AOS1, SUA1, UBLE1A
Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Cellular Location Nucleus.
Tissue Location Expression level increases during S phase and drops in G2 phase (at protein level)
Research Areas
Citations (0)

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The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.


Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999).
Gong, L., et al., FEBS Lett. 448(1):185-189 (1999).
Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).

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$ 295.00
$ 99.00
Cat# AP2511b
(40 western blots)
Availability: In Stock
Bulk Size
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