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SAE1 (AOS1) Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2511b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
SAE1 (AOS1) Antibody (C-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q9UBE0 |
| Other Accession | Q6AXQ0, Q9R1T2, A2VE14 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 38450 Da |
SAE1 (AOS1) Antibody (C-term) - Additional info | |
| Gene ID 10055 | |
| Other Names SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A | |
| Target/Specificity This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307~337 amino acids from the C-terminal region of human AOS1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SAE1 (AOS1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SAE1 (AOS1) Antibody (C-term) - Protein Information | |
| Name SAE1 | |
| Synonyms AOS1, SUA1, UBLE1A | |
| Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 | |
| Cellular Location Nucleus. | |
| Tissue Location Expression level increases during S phase and drops in G2 phase (at protein level) | |
SAE1 (AOS1) Antibody (C-term) - Related products
AP1199a: SAE1 (AOS1) Antibody (N-term)
AP1199b: SAE1 (AOS1) Antibody (C-term)
AP2011b: SAE1 (AOS1) Antibody (C-term)
AP2511a: SAE1 (AOS1) Antibody (N-term)
AP2511b: SAE1 (AOS1) Antibody (C-term)
DC00151: Human SAE1 cDNA Clone
LY10831a: SAE1 Over-expression Lysate
BP1199a: SAE1 Antibody (N-term) Blocking Peptide
BP1199b: SAE1 Antibody (C-term) Blocking Peptide
BP2011b: SAE1 Antibody (C-term) Blocking Peptide
BP2511a: AOS1 Antibody (N-term) Blocking Peptide
BP2511b: AOS1 Antibody (C-term) Blocking Peptide
SAE1 (AOS1) Antibody (C-term) - Application data
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The AOS1 C-term Pab (Cat. #AP2511b) is used in Western blot to detect AOS1 in mouse heart tissue lysate.
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Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (Cat.#AP2511b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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AOS1 Antibody (V315) (Cat. #AP2511b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).
SAE1 (AOS1) Antibody (C-term) - Research Areas
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BACKGROUND
The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
REFERENCES
Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999). Gong, L., et al., FEBS Lett. 448(1):185-189 (1999). Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).
