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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   M Sema5a Antibody   

M Sema5a Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - M Sema5a Antibody AP2712b
    Anti-Semaphorin 5A (C-term) at 1:1000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 121 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - M Sema5a Antibody AP2712b
    Western blot analysis of mouse anti-Semaphorin 5A Pab (Cat.#AP2712b) in mouse brain tissue lysates (35ug/lane). Semaphorin 5A(arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13591
Other Accession D3ZTD8, Q62217
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 120615 Da
Antigen Region 1006-1035 aa
Additional Information
Gene ID 9037
Other Names Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF
Target/Specificity This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1006-1035 amino acids from mouse Sema5a.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM Sema5a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms SEMAF
Function Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development.


Bialecka,M., Neurosci. Lett. 399 (1-2), 121-123 (2006)
Melin,M., Neuropsychobiology 54 (1), 64-69 (2006)

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$ 295.00
$ 99.00
Cat# AP2712b
(40 western blots)
Availability: In Stock
Bulk Size
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