|Application ||WB, IHC-P, E|
|Calculated MW||123473 Da|
|Antigen Region||45-74 aa|
|Other Names||Disheveled-associated activator of morphogenesis 1, DAAM1, KIAA0666|
|Target/Specificity||This DAAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human DAAM1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DAAM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to disheveled (Dvl) and Rho, and mediates Wnt- induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208). Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, cilium basal body. Note=Perinuclear|
|Tissue Location||Expressed in all tissues examined.|
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Provided below are standard protocols that you may find useful for product applications.
Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton. Recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.
Liu,W., Proc. Natl. Acad. Sci. U.S.A. 105 (1), 210-215 (2008)
Yamashita,M.,Genes Cells 12 (11), 1255-1265 (2007)
Lu,J., J. Mol. Biol. 369 (5), 1258-1269 (2007)
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