|Application ||IHC-P, WB, E|
|Other Accession||O70367, O77770, Q9Z1F6, P17404|
|Predicted||Bovine, Mouse, Rabbit, Rat|
|Calculated MW||37102 Da|
|Antigen Region||253-281 aa|
|Other Names||Leukocyte cell-derived chemotaxin 1, Chondrosurfactant protein, CH-SP, Chondromodulin-1, Chondromodulin-I, ChM-I, LECT1, CHMI|
|Target/Specificity||This LECT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the C-terminal region of human LECT1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LECT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development. Inhibits in vitro tube formation and mobilization of endothelial cells. Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization.|
|Cellular Location||Chondromodulin-1: Secreted, extracellular space, extracellular matrix. Note=Accumulated in the inter- territorial matrix of cartilage|
|Tissue Location||Detected in cartilage and cardiac valves (at protein level). Detected in the laminae fibrosa, spongiosa and ventricularis layers of normal cardiac valves (at protein level) Expression is decreased cardiac valves of patients with valvular heart disease (at protein level). Weakly expressed in chondrosarcoma.|
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Provided below are standard protocols that you may find useful for product applications.
LECT1 a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This protein is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development.
Aoyama,T., Biochem. Biophys. Res. Commun. 365 (1), 124-130 (2008)
Yoshioka,M., Nat. Med. 12 (10), 1151-1159 (2006)
Aoyama,T., J. Biol. Chem. 279 (27), 28789-28797 (2004)
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