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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Vimentin Antibody (S82)   

Vimentin Antibody (S82)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IF - Vimentin Antibody (S82) AP2739a
    Fluorescent confocal image of SY5Y cells stained with Vimentin (S82) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2739a Vimentin (S82) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (
  • WB - Vimentin Antibody (S82) AP2739a
    Western blot analysis of Vimentin-S82 (Cat. #AP2739a) in Hela cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.
  • WB - Vimentin Antibody (S82) AP2739a
    Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82) (Cat.#AP2739a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
  • IHC-P - Vimentin Antibody (S82) AP2739a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) (Cat.#AP2739a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08670
Other Accession P31000, P20152, Q4R4X4, P48670, P48616, Q9MZA9
Reactivity Human, Mouse
Predicted Bovine, Hamster, Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53652 Da
Antigen Region 63-90 aa
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Target/Specificity This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.
Dilution IF~~1:200
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVimentin Antibody (S82) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
Citations (0)

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Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.


References for protein:
1.Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008)
2. Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008)
3. Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704]

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$ 295.00
$ 99.00
Cat# AP2739a
(40 western blots)
Availability: In Stock
Bulk Size
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