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COL9A1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - COL9A1 Antibody (Center) AP2762c
    All lanes : Anti-COL9A1 Antibody (Center) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - COL9A1 Antibody (Center) AP2762c
    COL9A1 Antibody (Center) (Cat.# AP2762c) western blot analysis in MCF-7 cell line and mouse eyeball,rat eyeball tissue lysates (35ug/lane).This demonstrates the COL9A1 antibody detected the COL9A1 protein (arrow).
  • IHC-P - COL9A1 Antibody (Center) AP2762c
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COL9A1 antibody (Center) (Cat.#AP2762c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20849
Other Accession Q05722
Reactivity Human, Mouse, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 91869 Da
Antigen Region 428-456 aa
Additional Information
Gene ID 1297
Other Names Collagen alpha-1(IX) chain, COL9A1
Target/Specificity This COL9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-456 amino acids from the Central region of human COL9A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOL9A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL9A1
Function Structural component of hyaline cartilage and vitreous of the eye.
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations (0)

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COL9A1 is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule,and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in the COL9A1 gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects.


Fresquet,M., J. Biol. Chem. 282 (48), 34634-34643 (2007)
Liu,L.Y., Yi Chuan 29 (4), 427-432 (2007)
Van Camp,G., Am. J. Hum. Genet. 79 (3), 449-457 (2006)
Sivakumaran,T.A., J. Assoc. Res. Otolaryngol. 7 (2), 160-172 (2006)

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$ 295.00
$ 99.00
Cat# AP2762c
(40 western blots)
Availability: In Stock
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