|Application ||WB, IHC-P, E|
|Other Accession||Q9ESI7, O88809|
|Calculated MW||49318 Da|
|Antigen Region||107-137 aa|
|Other Names||Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX, DBCN, LISX|
|Target/Specificity||This DCX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-137 amino acids from human DCX.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DCX Antibody (S128) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.|
|Cellular Location||Cytoplasm. Cell projection. Note=Localizes at neurite tips.|
|Tissue Location||Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.
Leger,P.L., Neurogenetics (2008)
Zhang,Y., Biochem. Biophys. Res. Commun. 363 (3), 694-700 (2007)
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