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B2M Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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United States
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Ordering Information
Catalog # SizeAvailability Price  
AP2771A 400 µl
(40 western blots)
In Stock
$ 265.00email & fax$ 251.75online
Add to cart
AP2771A-ev 80 µl
(8 western blots)
In Stock
$ 95.00email & fax$ 90.25online
Add to cart
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B2M Antibody (N-term) - Product info

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC
Primary AccessionP61769
ReactivityHuman
Isotype Rabbit Ig
Calculated MW 13715 Da

B2M Antibody (N-term) - Additional info

Gene ID 567
Other Names
B2M; Beta-2-microglobulin; Beta-2-microglobulin form pI 5.3
Target/Specificity
This B2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-39 amino acids from the N-terminal region of human B2M.
Dilution
WB~~1:1000
FC~~1:10~50
IHC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
B2M Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

B2M Antibody (N-term) - Protein Information

Name B2M
Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Cellular Location
Secreted. Note=Detected in serum and urine.

B2M Antibody (N-term) - Related products

AM2052a: B2M Antibody (Ascites)

AM2052b: B2M Antibody

AP2771b: B2M Antibody (C-term)

AP2771d: B2M Antibody (C-term Y86)

BP2771a: B2M Antibody (N-term) Blocking Peptide

BP2771b: B2M Antibody (C-term) Blocking Peptide

BP2771d: B2M Antibody (C-term Y86) Blocking Peptide

AT1251a: B2M Antibody (monoclonal) (M01)

AJ1088a: Beta-2-Microglobulin Antibody

AO1976a: B2M Antibody

AO1980a: B2M Antibody

AF3768a: beta-2-microglobulin Antibody (N-Term)

AF3769a: beta-2-microglobulin (aa65-79) Antibody (internal region)

B2M Antibody (N-term) - Research Areas

Application data

  • WB - B2M Antibody (N-term) AP2771A

    Western blot analysis of B2M (arrow) using rabbit polyclonal B2M Antibody (N-term) (RB14679). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B2M gene (Lane 2) (Origene Technologies).

  • FC - B2M Antibody (N-term) AP2771A

    B2M Antibody (N-term) (Cat. #AP2771a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • IHC - B2M Antibody (N-term) AP2771A

    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (N-term) (Cat.#AP2771a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • WB - B2M Antibody (N-term) AP2771A
  • FC - B2M Antibody (N-term) AP2771A
  • IHC - B2M Antibody (N-term) AP2771A

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells.

REFERENCES

Sakata,M., J. Mol. Biol. 382 (5), 1242-1255 (2008)
Huang,W.C., Clin. Cancer Res. 14 (17), 5341-5347 (2008)
Gattoni-Celli,S., Cancer Res. 52 (5), 1201-1204 (1992)