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B2M Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - B2M Antibody (N-term) AP2771A
    Western blot analysis of B2M (arrow) using rabbit polyclonal B2M Antibody (N-term) (RB14679). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B2M gene (Lane 2) (Origene Technologies).
  • FC - B2M Antibody (N-term) AP2771A
    B2M Antibody (N-term) (Cat. #AP2771a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IHC-P - B2M Antibody (N-term) AP2771A
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (N-term) (Cat.#AP2771a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P61769
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13715 Da
Antigen Region 8-39 aa
Additional Information
Gene ID 567
Other Names Beta-2-microglobulin, Beta-2-microglobulin form pI 53, B2M
Target/Specificity This B2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-39 amino acids from the N-terminal region of human B2M.
Dilution WB~~1:1000
FC~~1:10~50
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB2M Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B2M
Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Cellular Location Secreted. Note=Detected in serum and urine
Research Areas
Citations (0)

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Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells.

References

Sakata,M., J. Mol. Biol. 382 (5), 1242-1255 (2008)
Huang,W.C., Clin. Cancer Res. 14 (17), 5341-5347 (2008)
Gattoni-Celli,S., Cancer Res. 52 (5), 1201-1204 (1992)

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$ 265.00
$ 95.00
Cat# AP2771A
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Quantity:
(40 western blots)
Availability: In Stock
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