|Application ||WB, IHC-P, E|
|Other Accession||P17183, P04764, P17182, NP_001966|
|Calculated MW||47269 Da|
|Antigen Region||6-32 aa|
|Other Names||Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2|
|Target/Specificity||This NSE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-32 amino acids from human NSE.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NSE Antibody (Y25) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).|
|Cellular Location||Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.|
|Tissue Location||The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Kotaska,K., Neuro Endocrinol. Lett. 28 (6), 761-764 (2007)
Forooghian,F., J. Clin. Immunol. 27 (4), 388-396 (2007)
Rech,T.H., Crit Care 10 (5), R133 (2006)
Oliva,D., Genomics 10 (1), 157-165 (1991)
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