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NSE Antibody (Y25)Peptide Affnity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2780b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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NSE Antibody (Y25) - Product info | |
| Application | WB, IHC
|
| Primary Accession | P09104 |
| Other Accession | P17183, P04764, P17182, NP_001966 |
| Reactivity | Human, Mouse |
| Predicted | Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 47269 Da |
NSE Antibody (Y25) - Additional info | |
| Gene ID 2026 | |
| Other Names ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase | |
| Target/Specificity This NSE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12~41 amino acids surrounding Y25 of human NSE. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions NSE Antibody (Y25) is for research use only and not for use in diagnostic or therapeutic procedures. | |
NSE Antibody (Y25) - Protein Information | |
| Name ENO2 | |
| Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity) | |
| Cellular Location Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity) | |
| Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons | |
NSE Antibody (Y25) - Related products
AP18121c: ENO2 Antibody (Center)
AP3589a: Phospho-NSE-pY236 Antibody
AP3727a: Phospho-NSE- Y25 Antibody
DC01092: Human ENO2 cDNA Clone
LY11490a: ENO2 Over-expression Lysate
BP2780a: NSE Antibody (Y236) Blocking Peptide
BP2780b: NSE Antibody (Y25) Blocking Peptide
BP3589a: Phospho-NSE-pY236 Antibody Blocking Peptide
BP3727a: Phospho-NSE-Y25 Antibody Blocking Peptide
NSE Antibody (Y25) - Application data
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Western blot analysis of NSE-Y25 Antibody (Cat.#AP2780b) in mouse cerebellum tissue and Y79, HepG2, Jurkat, CEM cell line lysates (35ug/lane). NSE (arrow) was detected using the purified Pab.
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NSE Antibody (Y25) (Cat. #AP2780b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSE Antibody (Y25) for immunohistochemistry. Clinical relevance has not been evaluated.
NSE Antibody (Y25) - Research Areas
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BACKGROUND
NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
REFERENCES
Kotaska,K., Neuro Endocrinol. Lett. 28 (6), 761-764 (2007) Forooghian,F., J. Clin. Immunol. 27 (4), 388-396 (2007) Rech,T.H., Crit Care 10 (5), R133 (2006) Oliva,D., Genomics 10 (1), 157-165 (1991)
