|Application ||WB, IHC-P, E|
|Other Accession||P17183, P04764, P17182, NP_001966|
|Calculated MW||47269 Da|
|Antigen Region||6-32 aa|
|Other Names||Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2|
|Target/Specificity||This NSE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-32 amino acids from human NSE.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NSE Antibody (Y25) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).|
|Cellular Location||Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.|
|Tissue Location||The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons|
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Provided below are standard protocols that you may find useful for product applications.
NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Kotaska,K., Neuro Endocrinol. Lett. 28 (6), 761-764 (2007)
Forooghian,F., J. Clin. Immunol. 27 (4), 388-396 (2007)
Rech,T.H., Crit Care 10 (5), R133 (2006)
Oliva,D., Genomics 10 (1), 157-165 (1991)
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