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MTHFD1 Antibody (Center P550)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2788f | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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MTHFD1 Antibody (Center P550) - Product info | |
| Application | IF, WB, IHC
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| Primary Accession | P11586 |
| Other Accession | P27653, Q922D8, NP_005947 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 101559 Da |
MTHFD1 Antibody (Center P550) - Additional info | |
| Gene ID 4522 | |
| Other Names MTHFD1; MTHFC; MTHFD; C-1-tetrahydrofolate synthase, cytoplasmic; Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase; Formyltetrahydrofolate synthetase | |
| Target/Specificity This MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541~571 amino acids from the Center(P550) region of human MTHFD1. | |
| Dilution IF~~1:10~50 WB~~1:50~100WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions MTHFD1 Antibody (Center P550) is for research use only and not for use in diagnostic or therapeutic procedures. | |
MTHFD1 Antibody (Center P550) - Protein Information | |
| Name MTHFD1 | |
| Synonyms MTHFC, MTHFD | |
| Cellular Location Cytoplasm. | |
| Tissue Location Ubiquitous. | |
MTHFD1 Antibody (Center P550) - Related products
AP2788f: MTHFD1 Antibody (Center P550)
RI13407: MTHFD1 predesign siRNA
MTHFD1 Antibody (Center P550) - Application data
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Confocal immunofluorescent analysis of MTHFD1 Antibody (Center P550) (Cat#AP2788f) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of MTHFD1 Antibody (Center P550) (Cat. #AP2788f) in Y79 cell line lysates (35ug/lane). MTHFD1 (arrow) was detected using the purified Pab.
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MTHFD1 Antibody (Center P550) (Cat. #AP2788f) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MTHFD1 Antibody (Center P550) for immunohistochemistry. Clinical relevance has not been evaluated.
MTHFD1 Antibody (Center P550) - Research Areas
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BACKGROUND
MTHFD1 is a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.
REFERENCES
Ivanov,A., J Am Diet Assoc 109 (2), 313-318 (2009) Schmidt A., Biochemistry 39:6325-6335(2000) Hol F.A., Clin. Genet. 53:119-125(1998)
