Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MTHFD1 Antibody (Center P550)   

MTHFD1 Antibody (Center P550)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MTHFD1 Antibody (Center P550) AP2788f
    All lanes : Anti-MTHFD1 Antibody (Center P550) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - MTHFD1 Antibody (Center P550) AP2788f
    Confocal immunofluorescent analysis of MTHFD1 Antibody (Center P550) (Cat#AP2788f) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • WB - MTHFD1 Antibody (Center P550) AP2788f
    Western blot analysis of MTHFD1 Antibody (Center P550) (Cat. #AP2788f) in Y79 cell line lysates (35ug/lane). MTHFD1 (arrow) was detected using the purified Pab.
  • IHC-P - MTHFD1 Antibody (Center P550) AP2788f
    MTHFD1 Antibody (Center P550) (Cat. #AP2788f) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MTHFD1 Antibody (Center P550) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11586
Other Accession P27653, Q922D8, NP_005947
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 101559 Da
Antigen Region 535-562 aa
Additional Information
Gene ID 4522
Other Names C-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, Formyltetrahydrofolate synthetase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, MTHFD1, MTHFC, MTHFD
Target/Specificity This MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MTHFD1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMTHFD1 Antibody (Center P550) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Cellular Location Cytoplasm.
Tissue Location Ubiquitous.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


MTHFD1 is a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.


Ivanov,A., J Am Diet Assoc 109 (2), 313-318 (2009)
Schmidt A., Biochemistry 39:6325-6335(2000)
Hol F.A., Clin. Genet. 53:119-125(1998)

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 295.00
$ 99.00
Cat# AP2788f
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions