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SFRS2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SFRS2 Antibody (Center) AP2800c
    Western blot analysis of SFRS2 Antibody (Center) (Cat. #AP2800c) in mouse stomach tissue lysates (35ug/lane). SFRS2 (arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q01130
Other Accession Q6PDU1, Q06A98, Q62093, P30352, Q3MHR5
Reactivity Human
Predicted Bovine, Chicken, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 25476 Da
Antigen Region 76-105 aa
Additional Information
Gene ID 6427
Other Names Serine/arginine-rich splicing factor 2, Protein PR264, Splicing component, 35 kDa, Splicing factor SC35, SC-35, Splicing factor, arginine/serine-rich 2, SRSF2, SFRS2
Target/Specificity This SFRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human SFRS2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSFRS2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SRSF2
Synonyms SFRS2
Function Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
Cellular Location Nucleus. Nucleus, nucleoplasm. Nucleus speckle. Note=Phosphorylation by SRPK2 provokes its redistribution from the nuclear specke to nucleoplasm
Citations (0)

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Background

SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.

References

Merdzhanova,G., Cell Death Differ. 15 (12), 1815-1823 (2008)
Solis,A.S., J. Biol. Chem. 283 (35), 23619-23626 (2008)
Donev,R., Mol. Psychiatry 12 (7), 681-690 (2007)
Sureau,A., Proc. Natl. Acad. Sci. U.S.A. 89 (24), 11683-11687 (1992)

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Cat# AP2800c
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(40 western blots)
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