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APEX1 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP2849a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
APEX1 Antibody (N-term) - Product info | |
| Application | WB, IF
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| Primary Accession | P27695 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 35555 Da |
APEX1 Antibody (N-term) - Additional info | |
| Gene ID 328 | |
| Other Names APEX1; APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; Apurinic-apyrimidinic endonuclease 1; REF-1; Redox factor-1; DNA-(apurinic or apyrimidinic site) lyase, mitochondrial | |
| Target/Specificity This APEX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~70 amino acids from the N-terminal region of human APEX1. | |
| Dilution WB~~1:100~500WB~~1:1000 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions APEX1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
APEX1 Antibody (N-term) - Protein Information | |
| Name APEX1 | |
| Synonyms APE, APE1, APEX, APX, HAP1, REF1 | |
| Function Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'- phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA | |
| Cellular Location Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Note=Detected in the cytoplasm of B-cells stimulated to switch (By similarity) Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling Ubiquitinated form is localized predominantly in the cytoplasm | |
APEX1 Antibody (N-term) - Related products
AP2849a: APEX1 Antibody (N-term)
AP2849c: APEX1 Antibody (Center)
RI10327: APEX1 predesign siRNA
DC00903: Human APEX1 cDNA Clone
LY10802a: APEX1 Over-expression Lysate
BP2849a: APEX1 Antibody (N-term) Blocking Peptide
BP2849c: APEX1 Antibody (Center) Blocking Peptide
APEX1 Antibody (N-term) - Application data
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Western blot analysis of anti-APEX1 Antibody (N-term) (Cat.#AP2849a) in Hela cell line lysates (35ug/lane). APEX1 (arrow) was detected using the purified Pab.
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Western blot analysis of APEX1 (arrow) using rabbit polyclonal APEX1 Antibody (N-term) (Cat.#AP2849a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.
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Confocal immunofluorescent analysis of APEX1 Antibody (N-term)(Cat#AP2849a) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
APEX1 Antibody (N-term) - Research Areas
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BACKGROUND
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This protein is the major AP endonuclease in human cells.
REFERENCES
Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009) Li,W.Q., Carcinogenesis 30 (3), 500-505 (2009) Lo,Y.L., Cancer Epidemiol. Biomarkers Prev. 18 (1), 223-229 (2009) Bhakat,K.K., EMBO J. 22 (23), 6299-6309 (2003)
