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HSPD1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - HSPD1 Antibody (Center) AP2859c
    HSPD1 Antibody (Center) (Cat. #AP2859c) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).
  • IF - HSPD1 Antibody (Center) AP2859c
    Fluorescent image of U251 cells stained with HSPD1 (Center) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2859C HSPD1 (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria, supported by Human Protein Atlas Data (
  • IF - HSPD1 Antibody (Center) AP2859c
    Fluorescent confocal image of Hela cell stained with HSPD1 Antibody (Center)(Cat#AP2859c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10809
Other Accession P63039, P63038, P18687, Q5ZL72, P31081
Reactivity Human
Predicted Bovine, Chicken, Hamster, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61055 Da
Antigen Region 187-215 aa
Additional Information
Gene ID 3329
Other Names 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Target/Specificity This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-215 amino acids from the Central region of human HSPD1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPD1
Synonyms HSP60
Function Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.


References for protein:
1.Venner T.J., Singh B., Gupta R.S.DNA Cell Biol. 9:545-552(1990)
2.Hansen J.J., Bross P., Westergaard M., Nielsen M.N., Eiberg H.,Hum. Genet. 112:71-77(2003)
3.Rasmussen R.K., Ji H., Eddes J.S., Moritz R.L.,Electrophoresis 18:588-598(1997)
4.Aboulaich N., Vainonen J.P., Stralfors P., Vener A.V.Biochem. J. 383:237-248(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].

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$ 295.00
$ 99.00
Cat# AP2859c
(40 western blots)
Availability: In Stock
Bulk Size
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