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POLD1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - POLD1 Antibody (Center) AP2867c
    Western blot analysis of POLD1 Antibody (Center) (Cat. #AP2867c) in Jurkat cell line lysates (35ug/lane). POLD1 (arrow) was detected using the purified Pab.
  • FC - POLD1 Antibody (Center) AP2867c
    POLD1 Antibody (Center) (Cat. #AP2867c) flow cytometry analysis of Jurkatcells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28340
Other Accession P52431
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 123631 Da
Antigen Region 710-739 aa
Additional Information
Gene ID 5424
Other Names DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, POLD1, POLD
Target/Specificity This POLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 710-739 amino acids from the Central region of human POLD1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLD1
Synonyms POLD
Function Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
Cellular Location Nucleus.
Tissue Location Expressed across a panel of tissues, with high levels of expression in heart and lung
Citations (0)

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The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.


Chung D.W., Zhang J.,Proc. Natl. Acad. Sci. U.S.A. 88:11197-11201(1991)
Yang C.-L., Chang L.-S.Nucleic Acids Res. 20:735-745(1992)
Li H., Xie B., Zhou Y., Rahmeh A.J. Biol. Chem. 281:14748-14755(2006)

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$ 295.00
$ 99.00
Cat# AP2867c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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