|Application ||WB, FC, E|
|Calculated MW||123631 Da|
|Antigen Region||710-739 aa|
|Other Names||DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, POLD1, POLD|
|Target/Specificity||This POLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 710-739 amino acids from the Central region of human POLD1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POLD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.|
|Tissue Location||Expressed across a panel of tissues, with high levels of expression in heart and lung|
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Provided below are standard protocols that you may find useful for product applications.
The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
Chung D.W., Zhang J.,Proc. Natl. Acad. Sci. U.S.A. 88:11197-11201(1991)
Yang C.-L., Chang L.-S.Nucleic Acids Res. 20:735-745(1992)
Li H., Xie B., Zhou Y., Rahmeh A.J. Biol. Chem. 281:14748-14755(2006)
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