|Application ||WB, IF, E|
|Calculated MW||94103 Da|
|Antigen Region||1-30 aa|
|Other Names||Exonuclease 1, hExo1, 31--, Exonuclease I, hExoI, EXO1, EXOI, HEX1|
|Target/Specificity||This EXO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EXO1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EXO1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.|
|Cellular Location||Nucleus Note=Colocalizes with PCNA to discrete nuclear foci in S-phase|
|Tissue Location||Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
EXO1 is a protein with 5' to 3' exonuclease activity as well as an RNase H activity. This protein is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination.
Tishkoff D.X., Amin N.S., Viars C.S.Cancer Res. 58:5027-5031(1998)
Lee B.-I., Nguyen L.H.,Nucleic Acids Res. 30:942-949(2002)
Alam N.A., Gorman P., Jaeger E.E.M.Cancer Genet. Cytogenet. 147:121-127(2003)
Knudsen N.O., Nielsen F.C., Vinther L.Nucleic Acids Res. 35:2609-2619(2007)
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