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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   CD166 Antibody (N-term)   

CD166 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CD166 Antibody (N-term) AP2882a
    Western blot analysis of CD166 antibody (N-term) (Cat. #AP2882a) in NCI-H460 cell line lysates (35ug/lane). CD166 (arrow) was detected using the purified Pab.
  • IHC-P - CD166 Antibody (N-term) AP2882a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with CD166 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CD166 Antibody (N-term) AP2882a
    CD166 Antibody (N-term) (Cat. #AP2882a) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13740
Other Accession O35112, O46651, Q61490, Q9BH13
Reactivity Human
Predicted Bovine, Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 65102 Da
Antigen Region 58-87 aa
Additional Information
Gene ID 214
Other Names CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, ALCAM, MEMD
Target/Specificity This CD166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human CD166.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD166 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MEMD
Function Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
Research Areas
Citations (0)

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CD166 is cell adhesion molecule that binds to CD6. The protein is involved in neurite extension by neurons via heterophilic and homophilic interactions. It may play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.


Kahlert,C., Br. J. Cancer 101 (3), 457-464 (2009)
Kulasingam,V., Int. J. Cancer 125 (1), 9-14 (2009)

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Cat# AP2882a
(40 western blots)
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