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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ERP29 Antibody (Center)   

ERP29 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ERP29 Antibody (Center) AP2902c
    Western blot analysis of ERP29 Antibody (Center) (Cat. #AP2902c) in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane). ERP29 (arrow) was detected using the purified Pab.
  • WB - ERP29 Antibody (Center) AP2902c
    Western blot analysis of ERP29 (arrow) using rabbit polyclonal ERP29 Antibody (Center) (Cat. #AP2902c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2) .
  • IHC-P - ERP29 Antibody (Center) AP2902c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERP29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ERP29 Antibody (Center) AP2902c
    ERP29 Antibody (Center) (Cat. #AP2902c) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P30040
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 151-179 aa
Additional Information
Other Names Endoplasmic reticulum resident protein 29, ERp29, Endoplasmic reticulum resident protein 28, ERp28, Endoplasmic reticulum resident protein 31, ERp31, ERP29, C12orf8, ERP28
Target/Specificity This ERP29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-179 amino acids from the Central region of human ERP29.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERP29 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERP29
Synonyms C12orf8, ERP28
Function Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location Ubiquitous. Mostly expressed in secretory tissues EMBL; X94910; CAA64397.1; -; mRNA EMBL; AA412124; -; NOT_ANNOTATED_CDS; mRNA EMBL; CR541667; CAG46468.1; -; mRNA EMBL; AC073575; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC101493; AAI01494.1; -; mRNA EMBL; BC101495; AAI01496.1; -; mRNA CCDS; CCDS44977.1; -. [P30040-2] CCDS; CCDS9158.1; -. [P30040-1] PIR; T09549; T09549 RefSeq; NP_001029197.1; NM_001034025.1. [P30040-2] RefSeq; NP_006808.1; NM_006817.3. [P30040-1] UniGene; Hs.75841; - PDB; 2QC7; X-ray; 2.90 A; A/B=34-261 PDBsum; 2QC7; - ProteinModelPortal; P30040; - SMR; P30040; - BioGrid; 116160; 18 IntAct; P30040; 11 MINT; MINT-5002525; - STRING; 9606.ENSP00000261735; - iPTMnet; P30040; - PhosphoSitePlus; P30040; - SwissPalm; P30040; - BioMuta; ERP29; - DMDM; 6015110; - OGP; P30040; - REPRODUCTION-2DPAGE; IPI00024911; - SWISS-2DPAGE; P30040; - EPD; P30040; - PaxDb; P30040; - PeptideAtlas; P30040; - PRIDE; P30040; - TopDownProteomics; P30040-1; -. [P30040-1] Ensembl; ENST00000261735; ENSP00000261735; ENSG00000089248. [P30040-1] Ensembl; ENST00000455836; ENSP00000412083; ENSG00000089248. [P30040-2] GeneID; 10961; - KEGG; hsa:10961; - UCSC; uc001ttl.1; human. [P30040-1] CTD; 10961; - DisGeNET; 10961; - GeneCards; ERP29; - HGNC; HGNC:13799; ERP29 HPA; HPA039363; - HPA; HPA039456; - MIM; 602287; gene neXtProt; NX_P30040; - OpenTargets; ENSG00000089248; - PharmGKB; PA25509; - eggNOG; ENOG410IX2F; Eukaryota eggNOG; ENOG4111I8S; LUCA GeneTree; ENSGT00390000018566; - HOGENOM; HOG000169611; - HOVERGEN; HBG051508; - InParanoid; P30040; - KO; K09586; - OMA; DGCIKEF; - OrthoDB; EOG091G0NH4; - PhylomeDB; P30040; - TreeFam; TF324701; - BioCyc; ZFISH:ENSG00000089248-MONOMER; - ChiTaRS; ERP29; human EvolutionaryTrace; P30040; - GeneWiki; ERP29; - GenomeRNAi; 10961; - PRO; PR:P30040; - Proteomes; UP000005640; Chromosome 12 Bgee; ENSG00000089248; - CleanEx; HS_ERP29; - ExpressionAtlas; P30040; baseline and differential Genevisible; P30040; HS GO; GO:0009986; C:cell surface; IDA:MGI GO; GO:0005783; C:endoplasmic reticulum; IDA:ParkinsonsUK-UCL GO; GO:0005788; C:endoplasmic reticulum lumen; NAS:ParkinsonsUK-UCL GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell GO; GO:0016020; C:membrane; IDA:UniProtKB GO; GO:0005790; C:smooth endoplasmic reticulum; ISS:ParkinsonsUK-UCL GO; GO:0030133; C:transport vesicle; ISS:ParkinsonsUK-UCL GO; GO:0051087; F:chaperone binding; ISS:ParkinsonsUK-UCL GO; GO:0042803; F:protein homodimerization activity; ISS:ParkinsonsUK-UCL GO; GO:0000187; P:activation of MAPK activity; IDA:ParkinsonsUK-UCL GO; GO:0006886; P:intracellular protein transport; NAS:ParkinsonsUK-UCL GO; GO:0010629; P:negative regulation of gene expression; IDA:ParkinsonsUK-UCL GO; GO:0050709; P:negative regulation of protein secretion; IDA:ParkinsonsUK-UCL GO; GO:0010628; P:positive regulation of gene expression; IDA:ParkinsonsUK-UCL GO; GO:0001934; P:positive regulation of protein phosphorylation; IDA:ParkinsonsUK-UCL GO; GO:0006457; P:protein folding; NAS:ParkinsonsUK-UCL GO; GO:0009306; P:protein secretion; IEA:InterPro GO; GO:0043335; P:protein unfolding; NAS:ParkinsonsUK-UCL GO; GO:1902235; P:regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; IEA:Ensembl CDD; cd00238; ERp29c; 1 Gene3D; 1.20.1150.12; -; 1 Gene3D; 3.40.30.10; -; 1 InterPro; IPR016855; ER_p29 InterPro; IPR011679; ER_p29_C InterPro; IPR012883; ERp29_N InterPro; IPR012336; Thioredoxin-like_fold Pfam; PF07749; ERp29; 1 Pfam; PF07912; ERp29_N; 1 PIRSF; PIRSF027352; ER_p29; 1 SUPFAM; SSF47933; SSF47933; 1 SUPFAM; SSF52833; SSF52833; 1 PROSITE; PS00014; ER_TARGET; 1 1: Evidence at protein level; 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Phosphoprotein; Reference proteome; Signal SIGNAL 1 32 CHAIN 33 261 Endoplasmic reticulum resident protein 29 /FTId=PRO_0000021197 MOTIF 258 261 Prevents secretion from ER {ECO:0000255|PROSITE-ProRule:PRU10138} MOD_RES 64 64 Phosphotyrosine; by PKDCC MOD_RES 66 66 Phosphotyrosine; by PKDCC VAR_SEQ 49 53 VIPKS -> IMVTS (in isoform 2) {ECO:0000303|Ref.2} /FTId=VSP_045680 VAR_SEQ 54 261 Missing (in isoform 2) {ECO:0000303|Ref.2} /FTId=VSP_045681 HELIX 45 49 {ECO:0000244|PDB:2QC7} HELIX 50 52 {ECO:0000244|PDB:2QC7} STRAND 54 60 {ECO:0000244|PDB:2QC7} HELIX 68 80 {ECO:0000244|PDB:2QC7} STRAND 86 91 {ECO:0000244|PDB:2QC7} STRAND 96 98 {ECO:0000244|PDB:2QC7} HELIX 102 107 {ECO:0000244|PDB:2QC7} HELIX 112 114 {ECO:0000244|PDB:2QC7} STRAND 116 122 {ECO:0000244|PDB:2QC7} HELIX 138 147 {ECO:0000244|PDB:2QC7} HELIX 159 170 {ECO:0000244|PDB:2QC7} HELIX 174 180 {ECO:0000244|PDB:2QC7} HELIX 183 187 {ECO:0000244|PDB:2QC7} HELIX 193 211 {ECO:0000244|PDB:2QC7} HELIX 217 230 {ECO:0000244|PDB:2QC7} HELIX 235 248 {ECO:0000244|PDB:2QC7} SEQUENCE 261 AA; 28993 MW; 76145006433A1983 CRC64; MAAAVPRAAF LSPLLPLLLG FLLLSAPHGG SGLHTKGALP LDTVTFYKVI PKSKFVLVKF DTQYPYGEKQ DEFKRLAENS ASSDDLLVAE VGISDYGDKL NMELSEKYKL DKESYPVFYL FRDGDFENPV PYTGAVKVGA IQRWLKGQGV YLGMPGCLPV YDALAGEFIR ASGVEARQAL LKQGQDNLSS VKETQKKWAE QYLKIMGKIL DQGEDFPASE MTRIARLIEK NKMSDGKKEE LQKSLNILTA FQKKGAEKEE L
Research Areas
Citations ( 0 )

Background

ERP29 shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.

References

Bambang,I.F., et.al., Exp. Cell Res. 315 (11), 1964-1974 (2009)

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$ 295.00
$ 99.00
Cat# AP2902c
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(40 western blots)
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