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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   P4HB Antibody (C-term)   

P4HB Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - P4HB Antibody (C-term) AP2911b
    Western blot analysis of P4HB Antibody (C-term) (Cat. #AP2911b) in CEM,HepG2,A2058 cell line lysates (35ug/lane). P4HB (arrow) was detected using the purified Pab.(2ug/ml)
  • IHC-P - P4HB Antibody (C-term) AP2911b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with P4HB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - P4HB Antibody (C-term) AP2911b
    Flow cytometric analysis of K562 cells using P4HB Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07237
Other Accession P04785, P09103, Q8R4U2, P05307
Reactivity Human
Predicted Bovine, Hamster, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57116 Da
Antigen Region 436-464 aa
Additional Information
Gene ID 5034
Other Names Protein disulfide-isomerase, PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55, P4HB, ERBA2L, PDI, PDIA1, PO4DB
Target/Specificity This P4HB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-464 amino acids from the C-terminal region of human P4HB.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsP4HB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name P4HB
Synonyms ERBA2L, PDI, PDIA1, PO4DB
Function This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein. Note=Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Research Areas
Citations (0)

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P4HB is a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.


Ewing,R.M.,, Mol. Syst. Biol. 3, 89 (2007)

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$ 295.00
$ 99.00
Cat# AP2911b
(40 western blots)
Availability: In Stock
Bulk Size
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