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PABPC1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PABPC1 Antibody (Center) AP2920c
    Western blot analysis of PABPC1 Antibody (Center) (Cat. #AP2920c) in NIH-3T3 cell line lysates (35ug/lane). PABPC1 (arrow) was detected using the purified Pab.
  • IHC-P - PABPC1 Antibody (Center) AP2920c
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PABPC1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P11940
Other Accession Q6IP09, P20965, Q9EPH8, P29341, P61286
Reactivity Human, Mouse
Predicted Bovine, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 70671 Da
Antigen Region 250-279 aa
Additional Information
Gene ID 26986
Other Names Polyadenylate-binding protein 1, PABP-1, Poly(A)-binding protein 1, PABPC1, PAB1, PABP1, PABPC2
Target/Specificity This PABPC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human PABPC1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPABPC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PABPC1
Synonyms PAB1, PABP1, PABPC2
Function Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA- binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding- region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed.
Cellular Location Cytoplasm. Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the cytoplasm and the nucleus
Tissue Location Ubiquitous.
Citations (0)

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Background

PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.

References

Le Clerc,S., et.al., J. Infect. Dis. 200 (8), 1194-1201 (2009)

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$ 295.00
$ 99.00
Cat# AP2920c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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