PHGDH Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, IHC-P, E |
---|---|
Primary Accession | O43175 |
Other Accession | A5GFY8, Q61753, Q60HD7, Q5EAD2 |
Reactivity | Human, Mouse |
Predicted | Bovine, Monkey, Pig |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 56651 Da |
Antigen Region | 140-168 aa |
Gene ID | 26227 |
---|---|
Other Names | D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3 |
Target/Specificity | This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the N-terminal region of human PHGDH. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PHGDH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PHGDH |
---|---|
Synonyms | PGDH3 |
Function | Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L- serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate. |
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Provided below are standard protocols that you may find useful for product applications.
Background
3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
References
Klomp,L.W., et.al., Am. J. Hum. Genet. 67 (6), 1389-1399 (2000)
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