- CITATIONS: 1
|Application ||WB, FC, E|
|Calculated MW||116181 Da|
|Antigen Region||986-1012 aa|
|Other Names||Klotho, Klotho peptide, KL|
|Target/Specificity||This Klotho antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 986-1012 amino acids from the C-terminal region of human Klotho.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Klotho Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=Isoform 1 shedding leads to a soluble peptide Klotho peptide: Secreted.|
|Tissue Location||Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney|
Provided below are standard protocols that you may find useful for product applications.
Klotho is a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.
Sugiyama,N., et.al., Mol. Cell Proteomics 6 (6), 1103-1109 (2007)
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