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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GOT1 Antibody (N-term)   

GOT1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GOT1 Antibody (N-term) AP2947A
    Anti-GOT1 Antibody (N-term) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GOT1 Antibody (N-term) AP2947A
    Western blot analysis of GOT1 Antibody (N-term) (Cat. #AP2947a) in mouse brain tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab.
  • WB - GOT1 Antibody (N-term) AP2947A
    Western blot analysis of GOT1 (arrow) using rabbit polyclonal GOT1 Antibody (N-term) (Cat. #AP2947a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GOT1 gene (Lane 2) .
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17174
Other Accession Q4R5L1, P33097, P08906
Reactivity Human, Mouse
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46248 Da
Antigen Region 5-33 aa
Additional Information
Gene ID 2805
Other Names Aspartate aminotransferase, cytoplasmic, cAspAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT, Glutamate oxaloacetate transaminase 1, Transaminase A, GOT1
Target/Specificity This GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human GOT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGOT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GOT1
Function Biosynthesis of L-glutamate from L-aspartate or L- cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3- mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.


Panteghini,M.,. Clin. Biochem. 23 (4), 311-319 (1990)
Doyle,J.M.,, Biochem. J. 270 (3), 651-657 (1990)

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$ 295.00
$ 99.00
Cat# AP2947A
(40 western blots)
Availability: In Stock
Bulk Size
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