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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GOT1 Antibody (C-term)   

GOT1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GOT1 Antibody (C-term) AP2947B
    Western blot analysis of GOT1 Antibody (C-term) (Cat. #AP2947b) in mouse liver tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab.
  • WB - GOT1 Antibody (C-term) AP2947B
    Western blot analysis of GOT1 (arrow) using rabbit polyclonal GOT1 Antibody (C-term) (Cat. #AP2947b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GOT1 gene.
  • IHC-P - GOT1 Antibody (C-term) AP2947B
    GOT1 Antibody (C-term) (Cat. #AP2947b) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GOT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - GOT1 Antibody (C-term) AP2947B
    GOT1 Antibody (C-term) (Cat. #AP2947b) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17174
Other Accession P13221, P05201
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46248 Da
Antigen Region 352-381 aa
Additional Information
Gene ID 2805
Other Names Aspartate aminotransferase, cytoplasmic, cAspAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT, Glutamate oxaloacetate transaminase 1, Transaminase A, GOT1
Target/Specificity This GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-381 amino acids from the C-terminal region of human GOT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGOT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GOT1
Function Biosynthesis of L-glutamate from L-aspartate or L- cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3- mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.


Franke, A., et al. Nat. Genet. 42(4):292-294(2010)
Barrett, J.C., et al. Nat. Genet. 41(12):1330-1334(2009)
Campos, J., et al. Eur. J. Intern. Med. 20 (3), E53-E56 (2009)

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Cat# AP2947B
(40 western blots)
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