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Phospho-CDC6(S54) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Phospho-CDC6(S54) Antibody AP3058a
    The anti-Phospho-CDC6-S54 Pab (Cat. #AP3058a) is used in Western blot to detect Phospho-CDC6-S54 in Ramos tissue lysate
  • IHC-P - Phospho-CDC6(S54) Antibody AP3058a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99741
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62720 Da
Additional Information
Gene ID 990
Other Names Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, HsCdc18, p62(cdc6), HsCDC6, CDC6, CDC18L
Target/Specificity This CDC6 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S54 of human CDC6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CDC6(S54) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC6
Synonyms CDC18L
Function Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Cellular Location Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells
Research Areas
Citations (0)

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The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.


Alexandrow, M.G., et al., Mol. Cell. Biol. 24(4):1614-1627 (2004).
Yim, H., et al., Mol. Biol. Cell 14(10):4250-4259 (2003).
Clay-Farrace, L., et al., EMBO J. 22(3):704-712 (2003).
Pelizon, C., et al., EMBO Rep. 3(8):780-784 (2002).
Bermejo, R., et al., Mol. Biol. Cell 13(11):3989-4000 (2002).

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$ 295.00
$ 99.00
Cat# AP3058a
(40 western blots)
Availability: In Stock
Bulk Size
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