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Phospho-Rb-like-1(S975) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Phospho-Rb-like-1(S975) Antibody AP3232a
    All lanes : Anti-Phospho-Rb-like-1(S975) Antibody (upper) at 1:250 dilution + Hela whole cell lysates, Beta-actin (lower) Lane 1: G0-phase(serum-deprived 24h) Lane 2: S-phase,hydroxycarbamide,5mM, 24h Lane 3: G2/M-phase,etoposide,5mM, 24h Lane 4: M-phase, nocodazole, 150ng/ml 20h Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 121 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Phospho-Rb-like-1(S975) Antibody AP3232a
    The anti-Phospho-Rb-like-1-S975 Pab (Cat. #AP3232a) is used in Western blot to detect Phospho-Rb-like-1-S975 in A2058 tissue lysate
  • IHC-P - Phospho-Rb-like-1(S975) Antibody AP3232a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28749
Other Accession Q64701
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 120847 Da
Additional Information
Gene ID 5933
Other Names Retinoblastoma-like protein 1, 107 kDa retinoblastoma-associated protein, p107, pRb1, RBL1
Target/Specificity This Rb-like-1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like-1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Rb-like-1(S975) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RBL1
Function Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
Cellular Location Nucleus.
Research Areas
Citations (0)

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RB-like 1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and RB-like 1 can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that RB-like 1 may also be a tumor suppressor.


Rodier, G., et al., J. Cell Biol. 168(1):55-66 (2005).
Barbie, T.U., et al., Proc. Natl. Acad. Sci. U.S.A. 100(26):15601-15606 (2003).
Joaquin, M., et al., J. Biol. Chem. 278(45):44255-44264 (2003).
Cicchillitti, L., et al., J. Biol. Chem. 278(21):19509-19517 (2003).
Leng, X., et al., Mol. Cell. Biol. 22(7):2242-2254 (2002).

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$ 295.00
$ 99.00
Cat# AP3232a
(40 western blots)
Availability: In Stock
Bulk Size
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