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> home > Products > Antibodies > Biological Process > Signal Transduction > Bi-phospho-ERK1/2-T202/Y204 Antibody

Bi-phospho-ERK1/2-T202/Y204 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Catalog #	Size	Availability	Price
AP3607a	0.1 mg 400 ul	In Stock	$ 255.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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Bi-phospho-ERK1/2-T202/Y204 Antibody - Product info
Application	DB, WB Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	P27361
Reactivity	Human
Concentration	0.25 mg/ml
Isotype	Rabbit Ig
Calculated MW	43136 Da
Bi-phospho-ERK1/2-T202/Y204 Antibody - Additional info
Gene ID 5595
Other Names MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; Microtubule-associated protein 2 kinase; p44-ERK1
Target/Specificity This Bi-phospho-ERK1/2-T202/Y204 antibody is generated from rabbits immunized with a KLH conjugated synthetic bisphosphopeptide incorporating amino acid residues surrounding phosphorylated T202 and Y204 of human ERK1.
Dilution DB~~1:500 WB~~1:100~500WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step 2-sites phosphospecific peptide affinity purification.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions Bi-phospho-ERK1/2-T202/Y204 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Bi-phospho-ERK1/2-T202/Y204 Antibody - Protein Information
Name MAPK3
Synonyms ERK1, PRKM3
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10) Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade
Cellular Location Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization

Bi-phospho-ERK1/2-T202/Y204 Antibody - Related products

AP3605a: Phospho-MAPK3-pT202 Antibody

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AP3607a: Bi-phospho-ERK1/2-T202/Y204 Antibody

AP7251a: MAPK3 Antibody (N-term)

AP7251b: MAPK3 Antibody (C-term)

AP7251d: MAPK3 Antibody (T202)

AP7251e: MAPK3 Antibody (Y204)

AP7251f: MAPK3 Antibody (D192)

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BP3605a: Phospho-ERK1-pT202 Antibody Blocking Peptide

BP3606a: Phospho-ERK1-pY204 Antibody Blocking Peptide

BP3607a: Phospho-ERK1-pT202/pY204 Antibody Blocking Peptide

BP7251a: MAPK3 Antibody (N-term) Blocking Peptide

BP7251b: MAPK3 Antibody (C-term) Blocking Peptide

BP7251d: MAPK3 Antibody (T202) Blocking Peptide

BP7251e: MAPK3 Antibody (Y204) Blocking Peptide

BP7251f: ERK1 Antibody (D192) Blocking Peptide

BP7500a: ERK1 Antibody (N-term) Blocking Peptide

AT2789a: MAPK3 Antibody (monoclonal) (M01)

AT2790a: MAPK3 Antibody (monoclonal) (M02)

AT2791a: MAPK3 Antibody (monoclonal) (M03)

AJ1271a: MAPK3 Antibody (C-term)

AJ1271b: Phospho-MAPK3-pT202/T204 Antibody

AJ1458a: MAPK3 Antibody (N-term)

AJ1602a: Phospho-Erk1 (pT202/pY204) / Erk2 (pT185/pY187) Antibody

AF1379a: MAPK3 Antibody

Bi-phospho-ERK1/2-T202/Y204 Antibody - Application data

Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody (Cat.#AP3607a) on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) (Cat.#AP3607a). HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane).
Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) (Cat.#AP3607a). HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane) .

Bi-phospho-ERK1/2-T202/Y204 Antibody - Research Areas

Antibodies Biological Process Signal Transduction Protein Amino Acid Phosphorylation Post-translational Protein Modification Phosphate Metabolic Process Phosphorus Metabolic Process Phosphorylation Protein Modification Process Biopolymer Modification Metabolic Process Primary Metabolic Process Protein Metabolic Process Cellular Protein Metabolic Process Cellular Metabolic Process Cellular Process Macromolecule Metabolic Process Intracellular Signaling Cascade Cellular Macromolecule Metabolic Process Interspecies Interaction Between Organisms Cell Cycle Multi-organism Process Biological Regulation Small GTPase Mediated Signal Transduction Regulation Of Biological Process Regulation Of Cellular Process Ras Protein Signal Transduction Cellular Compartment Cytoplasm Intracellular Part Intracellular Cytoplasmic Part Membrane-bounded Organelle Intracellular Membrane-bounded Organelle Membrane-enclosed Lumen Organelle Lumen Cytosol Organelle Intracellular Organelle Intracellular Organelle Lumen Pathway KEGG Cell Cycle

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.

REFERENCES

Munshi, H.G., et al., J. Biol. Chem. 279(37):39042-39050 (2004). Mukherjee, S., et al., Infect. Immun. 72(9):5274-5282 (2004). Sebkova, L., et al., Infect. Immun. 72(9):5019-5026 (2004). Huang, H.M., et al., Biochem. Biophys. Res. Commun. 320(4):1247-1252 (2004). Mizuno, S., et al., Am. J. Respir. Cell Mol. Biol. 31(2):184-192 (2004).