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Phospho-JMJD2B(S622) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • DB - Phospho-JMJD2B(S622) Antibody AP3723a
    Dot blot analysis of anti-Phospho-JMJD2B-pS622 Phospho-specific Pab (Cat. #AP3723a ) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O94953
Other Accession Q91VY5
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 121897 Da
Additional Information
Gene ID 23030
Other Names Lysine-specific demethylase 4B, 11411-, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, KDM4B, JHDM3B, JMJD2B, KIAA0876
Target/Specificity This JMJD2B Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S622 of human JMJD2B.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-JMJD2B(S622) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM4B
Synonyms JHDM3B, JMJD2B, KIAA0876
Function Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00537, ECO:0000269|PubMed:15927959}
Research Areas
Citations (0)

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JMJD2B is histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. This protein does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. It is only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. It is demethylation of Lys residue generates formaldehyde and succinate.


Beyer, S., et al. J. Biol. Chem. 283(52):36542-36552(2008)
Pollard, P.J., et al. Biochem. J. 416(3):387-394(2008)
Katoh, Y., et al. Int. J. Mol. Med. 20(2):269-273(2007)

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Cat# AP3723a
(40 western blots)
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