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Phospho-JMJD2A(Y547) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • DB - Phospho-JMJD2A(Y547) Antibody AP3726a
    Dot blot analysis of anti-Phospho-JMJD2A-Y547 Phospho-specific Pab (Cat. #AP3726a ) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75164
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 120662 Da
Additional Information
Gene ID 9682
Other Names Lysine-specific demethylase 4A, 11411-, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A, KDM4A, JHDM3A, JMJD2, JMJD2A, KIAA0677
Target/Specificity This JMJD2A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y547 of human JMJD2A.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-JMJD2A(Y547) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM4A
Synonyms JHDM3A, JMJD2, JMJD2A, KIAA0677
Function Histone demethylase that specifically demethylates 'Lys- 9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00537, ECO:0000269|PubMed:15927959, ECO:0000269|PubMed:16024779}
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.


Trojer, P., et al. J. Biol. Chem. 284(13):8395-8405(2009)
Lee, J., et al. Nat. Struct. Mol. Biol. 15(1):109-111(2008)
Katoh, Y., et al. Int. J. Mol. Med. 20(2):269-273(2007)

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$ 295.00
$ 99.00
Cat# AP3726a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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