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Phospho-mouse JUN(T289) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • DB - Phospho-mouse JUN(T289) Antibody AP3783b
    Dot blot analysis of Phospho-mouse JUN-T289 Antibody Phospho-specific Pab (Cat. #AP3783b) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05627
Other Accession P17325, P56432, P05412, P18870, O77627, NP_002219.1
Reactivity Mouse
Predicted Bovine, Chicken, Human, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35944 Da
Additional Information
Gene ID 16476
Other Names Transcription factor AP-1, AH119, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, Jun A, Jun
Target/Specificity This mouse JUN Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T289 of mouse JUN.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-mouse JUN(T289) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Jun
Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Cellular Location Nucleus {ECO:0000250|UniProtKB:P05412}.
Research Areas
Citations (0)

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This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.


Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010)
Bozec, A., et al. J. Cell Biol. 190(6):1093-1106(2010)
Machida, K., et al. Hepatology 52(2):480-492(2010)
Madi, A., et al. BMC Microbiol. 10, 215 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :

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Cat# AP3783b
(40 western blots)
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