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Phospho-ARHGAP35(Y1105) antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Phospho-ARHGAP35(Y1105) antibody AP3903a
    Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-HUMAN-ARHGAP35(Y1105)(Cat. #AP3903a)(upper) or Beta-actin (lower).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NRY4
Other Accession P81128, Q91YM2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 170514 Da
Additional Information
Gene ID 2909
Other Names Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, GRF-1, Rho GAP p190A, p190-A, ARHGAP35, GRF1, GRLF1, KIAA1722
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1090-1135 amino acids from human.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-ARHGAP35(Y1105) antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARHGAP35 (HGNC:4591)
Function Rho GTPase-activating protein (GAP), binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase- activating protein (GAP) (By similarity). Transduces SRC-dependent signal from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F- actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed:1894621).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q91YM2}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q91YM2}. Cell membrane {ECO:0000250|UniProtKB:Q91YM2}. Nucleus. Note=In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin {ECO:0000250|UniProtKB:Q91YM2}
Tissue Location Detected in neutrophils (at protein level).
Research Areas
Citations (0)

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Background

Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'- GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.

References

Nagase T.,et al.DNA Res. 7:347-355(2000).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Tikoo A.,et al.Gene 257:23-31(2000).
LeClerc S.,et al.J. Biol. Chem. 266:17333-17340(1991).

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$ 295.00
$ 99.00
Cat# AP3903a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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