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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ANKFY1 Antibody (C-term)   

ANKFY1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ANKFY1 Antibody (C-term) AP4704b
    ANKFY1 Antibody (C-term) (Cat. #AP4704b) western blot analysis in MDA-MB435,CEM,MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the ANKFY1 antibody detected the ANKFY1 protein (arrow).
  • IHC-P - ANKFY1 Antibody (C-term) AP4704b
    ANKFY1 Antibody (C-term) (Cat. #AP4704b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKFY1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ANKFY1 Antibody (C-term) AP4704b
    ANKFY1 Antibody (C-term) (Cat. #AP4704b) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9P2R3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 128399 Da
Antigen Region 436-465 aa
Additional Information
Gene ID 51479
Other Names Rabankyrin-5, Rank-5, Ankyrin repeat and FYVE domain-containing protein 1, Ankyrin repeats hooked to a zinc finger motif, ANKFY1, ANKHZN, KIAA1255
Target/Specificity This ANKFY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-465 amino acids from the C-terminal region of human ANKFY1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANKFY1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANKFY1
Synonyms ANKHZN, KIAA1255
Function Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI(3)P). Involved in homotypic early endosome fusion and to a lesser extent in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Involved in macropinocytosis; the function is dependent on Rab5-GTP. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the subcellular localization of the retromer complex in a EHD1- dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport.
Cellular Location Cytoplasm. Endosome membrane; Peripheral membrane protein. Early endosome. Note=Also associated with endosomal membranes. Localizes to macropinosomes
Tissue Location High expression in whole adult brain and intermediate expression in all other tissues and specific brain regions examined, including fetal brain
Research Areas
Citations ( 0 )

Background

ANKFY1 encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport.

References

Bouslam, N., et al. Hum. Genet. 121 (3-4), 413-420 (2007)
Schnatwinkel, C., et al. PLoS Biol. 2 (9), E261 (2004)

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$ 295.00
$ 99.00
Cat# AP4704b
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Quantity:
(40 western blots)
Availability: In Stock
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