|Application ||WB, IHC-P, FC, E|
|Calculated MW||128399 Da|
|Antigen Region||436-465 aa|
|Other Names||Rabankyrin-5, Rank-5, Ankyrin repeat and FYVE domain-containing protein 1, Ankyrin repeats hooked to a zinc finger motif, ANKFY1, ANKHZN, KIAA1255|
|Target/Specificity||This ANKFY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-465 amino acids from the C-terminal region of human ANKFY1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ANKFY1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI(3)P). Involved in homotypic early endosome fusion and to a lesser extent in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Involved in macropinocytosis; the function is dependent on Rab5-GTP. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the subcellular localization of the retromer complex in a EHD1- dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport.|
|Cellular Location||Cytoplasm. Endosome membrane; Peripheral membrane protein. Early endosome. Note=Also associated with endosomal membranes. Localizes to macropinosomes|
|Tissue Location||High expression in whole adult brain and intermediate expression in all other tissues and specific brain regions examined, including fetal brain|
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Provided below are standard protocols that you may find useful for product applications.
ANKFY1 encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport.
Bouslam, N., et al. Hum. Genet. 121 (3-4), 413-420 (2007)
Schnatwinkel, C., et al. PLoS Biol. 2 (9), E261 (2004)
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