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PNPLA8 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, WB, E |
|---|---|
| Primary Accession | Q9NP80 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 88477 Da |
| Antigen Region | 212-240 aa |
| Gene ID | 50640 |
|---|---|
| Other Names | Calcium-independent phospholipase A2-gamma, Intracellular membrane-associated calcium-independent phospholipase A2 gamma, iPLA2-gamma, PNPLA-gamma, Patatin-like phospholipase domain-containing protein 8, iPLA2-2, PNPLA8, IPLA22, IPLA2G |
| Target/Specificity | This PNPLA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-240 amino acids from the N-terminal region of human PNPLA8. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PNPLA8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PNPLA8 (HGNC:28900) |
|---|---|
| Synonyms | IPLA22, IPLA2G |
| Function | Calcium-independent and membrane-bound phospholipase, that catalyzes the esterolytic cleavage of fatty acids from glycerophospholipids to yield free fatty acids and lysophospholipids, hence regulating membrane physical properties and the release of lipid second messengers and growth factors (PubMed:10833412, PubMed:10744668, PubMed:15695510, PubMed:15908428, PubMed:17213206, PubMed:18171998, PubMed:28442572). Hydrolyzes phosphatidylethanolamine, phosphatidylcholine and probably phosphatidylinositol with a possible preference for the former (PubMed:15695510). Has also a broad substrate specificity in terms of fatty acid moieties, hydrolyzing saturated and mono-unsaturated fatty acids at nearly equal rates from either the sn-1 or sn-2 position in diacyl phosphatidylcholine (PubMed:10833412, PubMed:10744668, PubMed:15695510, PubMed:15908428). However, has a weak activity toward polyunsaturated fatty acids at the sn-2 position, and thereby favors the production of 2-arachidonoyl lysophosphatidylcholine, a key branch point metabolite in eicosanoid signaling (PubMed:15908428). On the other hand, can produce arachidonic acid from the sn-1 position of diacyl phospholipid and from the sn-2 position of arachidonate-containing plasmalogen substrates (PubMed:15908428). Therefore, plays an important role in the mobilization of arachidonic acid in response to cellular stimuli and the generation of lipid second messengers (PubMed:15695510, PubMed:15908428). Can also hydrolyze lysophosphatidylcholine (PubMed:15695510). In the mitochondrial compartment, catalyzes the hydrolysis and release of oxidized aliphatic chains from cardiolipin and integrates mitochondrial bioenergetics and signaling. It is essential for maintaining efficient bioenergetic mitochondrial function through tailoring mitochondrial membrane lipid metabolism and composition (PubMed:28442572). |
| Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q5XTS1}; Single-pass membrane protein Mitochondrion membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein |
| Tissue Location | Expressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver and pancreas. Also expressed in bronchial epithelial cells and kidney. Highest expression is observed in skeletal muscle and heart. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
PNPLA8 catalyzes cleavage of fatty acids from phospholipids, thereby regulating membrane physical properties and the release of lipid second messengers and growth factors. Phospholipase A2 activity also modulates cellular growth programs, inflammation, and ion channel function.
References
Harper, M.T., et al. J. Thromb. Haemost. 6(10):1819-1821(2008)
Wilson, P.A., et al. J. Lipid Res. 47(9):1940-1949(2006)
Murakami, M., et al. J. Biol. Chem. 280(14):14028-14041(2005)
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$ 365.00
$ 140.00
Cat# AP4706a
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