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PAR6G Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PAR6G Antibody (N-term) AP4750A
    Western blot analysis of PAR6G Antibody (N-term) (Cat. #AP4750a) in ZR-75-1 cell line lysates (35ug/lane). PAR6G (arrow) was detected using the purified Pab.
  • IHC-P - PAR6G Antibody (N-term) AP4750A
    PAR6G Antibody (N-term) (Cat. #AP4750a) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAR6G Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9BYG4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40883 Da
Antigen Region 18-47 aa
Additional Information
Gene ID 84552
Other Names Partitioning defective 6 homolog gamma, PAR-6 gamma, PAR6D, PARD6G, PAR6G
Target/Specificity This PAR6G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-47 amino acids from the N-terminal region of human PAR6G.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAR6G Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PARD6G
Synonyms PAR6G
Function Adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions. The PARD6-PARD3 complex links GTP- bound Rho small GTPases to atypical protein kinase C proteins (By similarity).
Cellular Location Cytoplasm. Cell membrane. Cell junction, tight junction
Tissue Location Widely expressed, with a higher expression in fetal and adult kidney
Research Areas
Citations (0)

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Background

PAR6G involved in asymmetrical cell division and cell polarization processes. PAR6G may play a role in the formation of epithelial tight junctions. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

References

Brajenovic, M., et al. J. Biol. Chem. 279(13):12804-12811(2004)
Kohjima, M., et al. Biochem. Biophys. Res. Commun. 299(4):641-646(2002)
Suzuki, A., et al. J. Cell Biol. 152(6):1183-1196(2001)

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Cat# AP4750A
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(40 western blots)
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