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FA2H Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - FA2H Antibody (Center) AP4799c
    Western blot analysis of FA2H Antibody (Center) (Cat. #AP4799c) in ZR-75-1 cell line lysates (35ug/lane). FA2H (arrow) was detected using the purified Pab.
  • WB - FA2H Antibody (Center) AP4799c
    Western blot analysis of FA2H Antibody (Center) (Cat. #AP4799c) in mouse brain tissue lysates (35ug/lane). FA2H (arrow) was detected using the purified Pab.
  • IHC-P - FA2H Antibody (Center) AP4799c
    FA2H Antibody (Center) (Cat. #AP4799c) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FA2H Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7L5A8
Other Accession Q2LAM0, Q5MPP0, Q4R4P4
Reactivity Human, Mouse
Predicted Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42791 Da
Antigen Region 121-148 aa
Additional Information
Gene ID 79152
Other Names Fatty acid 2-hydroxylase, 1---, Fatty acid alpha-hydroxylase, FA2H, FAAH
Target/Specificity This FA2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-148 amino acids from the Central region of human FA2H.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFA2H Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FA2H
Synonyms FAAH
Function Required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein
Tissue Location Detected in differentiating cultured keratinocytes (at protein level). Detected in epidermis and cultured keratinocytes. Highly expressed in brain and colon Detected at lower levels in testis, prostate, pancreas and kidney
Research Areas
Citations (0)

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FA2H is a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups.


Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009)
Edvardson, S., et al. Am. J. Hum. Genet. 83(5):643-648(2008)
Uchida, Y., et al. J. Biol. Chem. 282(18):13211-13219(2007)

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Cat# AP4799c
(40 western blots)
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