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DDX23 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DDX23 Antibody (Center) AP4866c
    Western blot analysis of DDX23 Antibody (Center) (Cat. #AP4866c) in 293 cell line lysates (35ug/lane). DDX23 (arrow) was detected using the purified Pab.
  • IHC-P - DDX23 Antibody (Center) AP4866c
    DDX23 Antibody (Center) (Cat. #AP4866c) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX23 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - DDX23 Antibody (Center) AP4866c
    DDX23 Antibody (Center) (Cat. #AP4866c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BUQ8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 95583 Da
Antigen Region 248-277 aa
Additional Information
Gene ID 9416
Other Names Probable ATP-dependent RNA helicase DDX23, 100 kDa U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD, DDX23
Target/Specificity This DDX23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-277 amino acids from the Central region of human DDX23.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDDX23 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDX23
Function Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.
Cellular Location Nucleus
Citations (0)

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DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.


Mathew, R., et al. Nat. Struct. Mol. Biol. 15(5):435-443(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 295.00
$ 99.00
Cat# AP4866c
(40 western blots)
Availability: In Stock
Bulk Size
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