|Application ||WB, IHC-P, FC, E|
|Calculated MW||95583 Da|
|Antigen Region||248-277 aa|
|Other Names||Probable ATP-dependent RNA helicase DDX23, 100 kDa U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD, DDX23|
|Target/Specificity||This DDX23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-277 amino acids from the Central region of human DDX23.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DDX23 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.|
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Provided below are standard protocols that you may find useful for product applications.
DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.
Mathew, R., et al. Nat. Struct. Mol. Biol. 15(5):435-443(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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