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HSP90B1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HSP90B1 Antibody (N-term) AP4899A
    HSP90B1 Antibody (N-term) (Cat. #AP4899a) western blot analysis in HepG2, CHO, K562, Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow).
  • IHC-P - HSP90B1 Antibody (N-term) AP4899A
    HSP90B1 Antibody (N-term) (Cat. #AP4899a) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSP90B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - HSP90B1 Antibody (N-term) AP4899A
    HSP90B1 Antibody (N-term) (Cat. #AP4899a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - HSP90B1 Antibody (N-term) AP4899A
    Confocal immunofluorescent analysis of HSP90B1 Antibody (N-term)(Cat#AP4899a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14625
Other Accession Q66HD0, Q29092, P08113, Q4R520, P08110, Q95M18
Reactivity Human, Hamster, Mouse
Predicted Bovine, Chicken, Monkey, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 92469 Da
Antigen Region 16-43 aa
Additional Information
Gene ID 7184
Other Names Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1
Target/Specificity This HSP90B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human HSP90B1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSP90B1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSP90B1
Synonyms GRP94, TRA1
Function Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
Citations (0)

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HSP90B1 is highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol.


Koo, B.H., et al. J. Biol. Chem. 285(1):197-205(2010) Suriano, R., et al. Glycobiology 19(12):1427-1435(2009) Lev, A., et al. J. Immunol. 183(7):4205-4210(2009)

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Cat# AP4899A
(40 western blots)
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