- CITATIONS: 2
|Application ||WB, IHC-P, E|
|Calculated MW||25619 Da|
|Antigen Region||110-138 aa|
|Other Names||N-acetyltransferase 8, 231-, Acetyltransferase 2, ATase2, Camello-like protein 1, Cysteinyl-conjugate N-acetyltransferase, CCNAT, NAT8 (HGNC:18069)|
|Target/Specificity||This NAT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the Central region of human NAT8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NAT8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acetylates the free alpha-amino group of cysteine S- conjugates to form mercapturic acids (PubMed:20392701). This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S- conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6- acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1 (PubMed:24556617). May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (PubMed:19011241).|
|Cellular Location||Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type II membrane protein Endoplasmic reticulum membrane; Single-pass type II membrane protein|
|Tissue Location||Preferentially expressed in liver and kidney. Also detected in brain (at protein level)|
Provided below are standard protocols that you may find useful for product applications.
This protein, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.
Ko, M.H., et al. J. Biol. Chem. 284(4):2482-2492(2009) Juhanson, P., et al. BMC Med. Genet. 9, 25 (2008) Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
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