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GRP78 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GRP78 Antibody AP50016
    Western blot analysis of lysates from Hela,HepG2 cell line (from left to right),using GRP78 Antibody(C0217). C0217 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • WB - GRP78 Antibody AP50016
    Western blot analysis of extracts from HUVEC cells (Lane 1), COS7 cells (Lane 2) and JK cells (Lane 3), using GRP78 Antibody. The lane on the left is treated with systhesized peptide.
  • IF - GRP78 Antibody AP50016
    Immunofluorescence analysis of COS7 cells, using GRP78 antibody .
  • IHC - GRP78 Antibody AP50016
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GRP78 antibody .
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession P11021
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 72 KDa
Antigen Region 623-654 aa
Additional Information
Gene ID 3309
Other Names 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78
Dilution WB~~ 1:1000
IF~~1:100
IHC~~1:50-1:100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name HSPA5
Synonyms GRP78
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Citations (0)

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Background

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

References

Ting J.,et al.DNA 7:275-286(1988).
Chao C.C.K.,et al.Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
Hansen J.J.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Bermudez-Fajardo A.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).

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$ 295.00
Cat# AP50016
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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